The detection of 3 ambiguous type 2 vaccine-derived polioviruses (VDPV2s) in Uganda

The detection of 3 ambiguous type 2 vaccine-derived polioviruses (VDPV2s) in Uganda

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Abstract Background The Oral Polio Vaccine (OPV or Sabin) is genetically unstable and may mutate to form vaccine-derived polioviruses (VDPVs).Methods In 2014, two VDPVs type 2 were identified during routine surveillance of acute baseball scoreboards for sale flaccid paralysis (AFP) cases.Consequently, a retrospective VDPV survey was conducted to ensure that there was no circulating VDPV in the country.

All Sabin poliovirus isolates identified in Uganda 6 months before and 6 months after were re-screened; Sabin 1 and 3 polioviruses were re-screened for Sabin 2 and Sabin 2 polioviruses were re-screened for VDPVs type 2.The Poliovirus rRT-PCR ITD/VDPV 4.0 assay and sequencing were used respectively.

Results read more The first two VDPVs type2 were identified in Eastern Uganda and the third was identified during the survey from South-western Uganda.These regions had low OPV coverage and poor AFP surveillance indicators.Conclusion The retrospective VDPV survey was a useful strategy to screen for VDPVs more exhaustively.

Supplementary surveillance methods need to be encouraged.